
Twenty-Third Annual Meeting and Symposium of the
Desert Tortoise Council, April 3-5, 1998
Abstracts

Plasma Corticosterone and Androstenedione in Male and Female
Desert Tortoises During the Reproductive Cycle
Valentine A. Lance1, David C. Rostal2 and Janice S. Grumbles2
1Center for Reproduction of Endangered Species, P.O. Box 551
San Diego, CA 92112
2Department of Biology, Georgia Southern University, Landrum Box
8042, Statesbro, GA 30460

Blood samples were collected by jugular puncture at monthly intervals
from April to October from 20 adult male and 30 adult female desert
tortoises. The tortoises were maintained in pens 15 m x 30 m
with natural vegetation and small artificial sod plots at the
Desert Tortoises Conservation Center, Las Vegas, Nevada. Water
and alfalfa hay were supplemented. Progesterone, testosterone,
estradiol, thyroid hormone, corticosterone and androstenedione
and lipids and calcium were analyzed in all of the plasma samples.
Part of this study was published earlier (Rostal et al. Herp.
Monogr. 8:72-82, 1994). Plasma corticosterone in male tortoises
ranged from 0.580 ng/ml to 5.990 ng/ml and showed a significant
seasonal pattern. Mean corticosterone in males was significantly
higher in August and September than in April, May and June. Female
values ranged from 0.200 ng/ml to 4.080 ng/ml. The mean monthly
values for female tortoises were significantly lower than that
of the males in every month, and the seasonal pattern was different.
Peak values in the females occurred in April, and lower mean
values were seen for all other months. Plasma androstenedione
in males showed a somewhat similar cycle in that higher values
were seen in August through October. Female samples are currently
being analyzed and will be presented.
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