From 1995 to 1997, two-hundred and thirty-seven blood samples
were collected from 234 gopher tortoises (Gopherus polyphemus)
on 42 sites in Florida to determine exposure to the pathogen (Mycoplasma
agassizii) that causes upper respiratory tract disease (URTD).
Twenty-seven (12%) of the gopher tortoises were seropositive,
9 (4%) were suspect, one was seronegative but culture-positive,
and the remaining 197 (84%) were seronegative. Of the three gopher
tortoises that were sampled twice, one remained seronegative and
the other two seroconverted from negative or suspect to positive.
The 27 seropositive gopher tortoises and one culture-positive
tortoise were from six locations: an old refuse dump, a state
park, and a military reservation in northern peninsular Florida;
an airport and a state park near Orlando; and a nature park in
St. Petersburg. Forty-six (20%) of the gopher tortoises showed
one or more clinical signs of URTD. Palpebral edema and ocular
discharge were the most common signs.